PubMed

Modification of the Tet-On regulatory system prevents the conditional-live HIV-1 variant from losing doxycycline-control.

Zhou X, Vink M, Berkhout B, Das AT.

Retrovirology. 2006 Nov 9;3:82.PMID: 17094796 [PubMed - indexed for MEDLINE]Related articlesFree article

The genetic stability of a conditional live HIV-1 variant can be improved by mutations in the Tet-On regulatory system that restrain evolution.

Zhou X, Vink M, Klaver B, Verhoef K, Marzio G, Das AT, Berkhout B.

J Biol Chem. 2006 Jun 23;281(25):17084-91. Epub 2006 Apr 20.PMID: 16627480 [PubMed - indexed for MEDLINE]Related articlesFree article

Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution.

Das AT, Klaver B, Centlivre M, Harwig A, Ooms M, Page M, Almond N, Yuan F, Piatak M Jr, Lifson JD, Berkhout B.

Retrovirology. 2008 Jun 5;5:44.PMID: 18533993 [PubMed - indexed for MEDLINE]Related articlesFree article

Optimization of the Tet-On system for regulated gene expression through viral evolution.

Zhou X, Vink M, Klaver B, Berkhout B, Das AT.

Gene Ther. 2006 Oct;13(19):1382-90. Epub 2006 May 25.PMID: 16724096 [PubMed - indexed for MEDLINE]Related articles

Strict control of human immunodeficiency virus type 1 replication by a genetic switch: Tet for Tat.

Verhoef K, Marzio G, Hillen W, Bujard H, Berkhout B.

J Virol. 2001 Jan;75(2):979-87.PMID: 11134311 [PubMed - indexed for MEDLINE]Related articlesFree article

Construction of a doxycycline-dependent simian immunodeficiency virus reveals a nontranscriptional function of tat in viral replication.

Das AT, Klaver B, Harwig A, Vink M, Ooms M, Centlivre M, Berkhout B.

J Virol. 2007 Oct;81(20):11159-69. Epub 2007 Aug 1.PMID: 17670816 [PubMed - indexed for MEDLINE]Related articlesFree article

Viral evolution as a tool to improve the tetracycline-regulated gene expression system.

Das AT, Zhou X, Vink M, Klaver B, Verhoef K, Marzio G, Berkhout B.

J Biol Chem. 2004 Apr 30;279(18):18776-82. Epub 2004 Feb 2.PMID: 14761948 [PubMed - indexed for MEDLINE]Related articlesFree article

Improving the safety of a conditional-live human immunodeficiency virus type 1 vaccine by controlling both gene expression and cell entry.

Das AT, Baldwin CE, Vink M, Berkhout B.

J Virol. 2005 Mar;79(6):3855-8.PMID: 15731280 [PubMed - indexed for MEDLINE]Related articlesFree article

Conditional virus replication as an approach to a safe live attenuated human immunodeficiency virus vaccine.

Berkhout B, Verhoef K, Marzio G, Klaver B, Vink M, Zhou X, Das AT.

J Neurovirol. 2002 Dec;8 Suppl 2:134-7. Review.PMID: 12491165 [PubMed - indexed for MEDLINE]Related articles

Dox-dependent SIVmac with tetracycline-inducible promoter in the U3 promoter region.

Xiao Y, Kuwata T, Miura T, Hayami M, Shida H.

Virology. 2000 Apr 10;269(2):268-75.PMID: 10753705 [PubMed - indexed for MEDLINE]Related articles

Construction of doxycyline-dependent mini-HIV-1 variants for the development of a virotherapy against leukemias.

Jeeninga RE, Jan B, van den Berg H, Berkhout B.

Retrovirology. 2006 Sep 27;3:64.PMID: 17005036 [PubMed - indexed for MEDLINE]Related articlesFree article

In vitro evolution of a highly replicating, doxycycline-dependent HIV for applications in vaccine studies.

Marzio G, Verhoef K, Vink M, Berkhout B.

Proc Natl Acad Sci U S A. 2001 May 22;98(11):6342-7. Epub 2001 May 15.PMID: 11353837 [PubMed - indexed for MEDLINE]Related articlesFree article

Improved single-chain transactivators of the Tet-On gene expression system.

Zhou X, Symons J, Hoppes R, Krueger C, Berens C, Hillen W, Berkhout B, Das AT.

BMC Biotechnol. 2007 Jan 19;7:6.PMID: 17239234 [PubMed - indexed for MEDLINE]Related articlesFree article

Control over HIV-1 replication by an antibiotic; a novel vaccination strategy with a drug-dependent virus.

Berkhout B, Marzio G, Verhoef K.

Virus Res. 2002 Jan 30;82(1-2):103-8. Review. No abstract available. PMID: 11885936 [PubMed - indexed for MEDLINE]Related articles

A second-site mutation that restores replication of a Tat-defective human immunodeficiency virus.

Verhoef K, Berkhout B.

J Virol. 1999 Apr;73(4):2781-9.PMID: 10074125 [PubMed - indexed for MEDLINE]Related articlesFree article

A conditionally replicating HIV-based vector that stably expresses an antiviral shRNA against HIV-1 replication.

Westerhout EM, Vink M, Haasnoot PC, Das AT, Berkhout B.

Mol Ther. 2006 Aug;14(2):268-75. Epub 2006 May 11.PMID: 16697708 [PubMed - indexed for MEDLINE]Related articles

Second site escape of a T20-dependent HIV-1 variant by a single amino acid change in the CD4 binding region of the envelope glycoprotein.

Baldwin CE, Berkhout B.

Retrovirology. 2006 Nov 29;3:84.PMID: 17134507 [PubMed - indexed for MEDLINE]Related articlesFree article

A bidirectional Tet-dependent promotor construct regulating the expression of E1A for tight control of oncolytic adenovirus replication.

Fechner H, Wang X, Picó AH, Wildner J, Suckau L, Pinkert S, Sipo I, Weger S, Poller W.

J Biotechnol. 2007 Jan 20;127(4):560-74. Epub 2006 Sep 27.PMID: 17083991 [PubMed - indexed for MEDLINE]Related articles

HIV-1 latency in actively dividing human T cell lines.

Jeeninga RE, Westerhout EM, van Gerven ML, Berkhout B.

Retrovirology. 2008 Apr 25;5:37.PMID: 18439275 [PubMed - indexed for MEDLINE]Related articlesFree article

Doxycycline-regulated lentiviral vector system with a novel reverse transactivator rtTA2S-M2 shows a tight control of gene expression in vitro and in vivo.

Koponen JK, Kankkonen H, Kannasto J, Wirth T, Hillen W, Bujard H, Ylä-Herttuala S.

Gene Ther. 2003 Mar;10(6):459-66.PMID: 12621450 [PubMed - indexed for MEDLINE]Related articles