PubMed

VACUTAINER CPT and Ficoll density gradient separation perform equivalently in maintaining the quality and function of PBMC from HIV seropositive blood samples.

Ruitenberg JJ, Mulder CB, Maino VC, Landay AL, Ghanekar SA.

BMC Immunol. 2006 May 25;7:11.PMID: 16725038 [PubMed - indexed for MEDLINE]Related articlesFree article

Optimal blood mononuclear cell isolation procedures for gamma interferon enzyme-linked immunospot testing of healthy Swedish and Tanzanian subjects.

Nilsson C, Aboud S, Karlén K, Hejdeman B, Urassa W, Biberfeld G.

Clin Vaccine Immunol. 2008 Apr;15(4):585-9. Epub 2008 Feb 20.PMID: 18287577 [PubMed - indexed for MEDLINE]Related articlesFree article

Enhanced ELISPOT detection of antigen-specific T cell responses from cryopreserved specimens with addition of both IL-7 and IL-15--the Amplispot assay.

Jennes W, Kestens L, Nixon DF, Shacklett BL.

J Immunol Methods. 2002 Dec 1;270(1):99-108.PMID: 12379342 [PubMed - indexed for MEDLINE]Related articles

Preservation of lymphocyte immunophenotype and proliferative responses in cryopreserved peripheral blood mononuclear cells from human immunodeficiency virus type 1-infected donors: implications for multicenter clinical trials. The ACTG Immunology Advanced Technology Laboratories.

Reimann KA, Chernoff M, Wilkening CL, Nickerson CE, Landay AL.

Clin Diagn Lab Immunol. 2000 May;7(3):352-9.PMID: 10799445 [PubMed - indexed for MEDLINE]Related articlesFree article

Standardization of cytokine flow cytometry assays.

Maecker HT, Rinfret A, D'Souza P, Darden J, Roig E, Landry C, Hayes P, Birungi J, Anzala O, Garcia M, Harari A, Frank I, Baydo R, Baker M, Holbrook J, Ottinger J, Lamoreaux L, Epling CL, Sinclair E, Suni MA, Punt K, Calarota S, El-Bahi S, Alter G, Maila H, Kuta E, Cox J, Gray C, Altfeld M, Nougarede N, Boyer J, Tussey L, Tobery T, Bredt B, Roederer M, Koup R, Maino VC, Weinhold K, Pantaleo G, Gilmour J, Horton H, Sekaly RP.

BMC Immunol. 2005 Jun 24;6:13.PMID: 15978127 [PubMed - indexed for MEDLINE]Related articlesFree article

Effect of shipment, storage, anticoagulant, and cell separation on lymphocyte proliferation assays for human immunodeficiency virus-infected patients.

Weinberg A, Betensky RA, Zhang L, Ray G.

Clin Diagn Lab Immunol. 1998 Nov;5(6):804-7.PMID: 9801338 [PubMed - indexed for MEDLINE]Related articlesFree article

Use of overlapping peptide mixtures as antigens for cytokine flow cytometry.

Maecker HT, Dunn HS, Suni MA, Khatamzas E, Pitcher CJ, Bunde T, Persaud N, Trigona W, Fu TM, Sinclair E, Bredt BM, McCune JM, Maino VC, Kern F, Picker LJ.

J Immunol Methods. 2001 Sep 1;255(1-2):27-40.PMID: 11470284 [PubMed - indexed for MEDLINE]Related articles

Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT.

Maecker HT, Moon J, Bhatia S, Ghanekar SA, Maino VC, Payne JK, Kuus-Reichel K, Chang JC, Summers A, Clay TM, Morse MA, Lyerly HK, DeLaRosa C, Ankerst DP, Disis ML.

BMC Immunol. 2005 Jul 18;6:17.PMID: 16026627 [PubMed - indexed for MEDLINE]Related articlesFree article

Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials.

Bull M, Lee D, Stucky J, Chiu YL, Rubin A, Horton H, McElrath MJ.

J Immunol Methods. 2007 Apr 30;322(1-2):57-69. Epub 2007 Feb 28.PMID: 17382342 [PubMed - indexed for MEDLINE]Related articlesFree article

Substantial improvements in performance indicators achieved in a peripheral blood mononuclear cell cryopreservation quality assurance program using single donor samples.

Dyer WB, Pett SL, Sullivan JS, Emery S, Cooper DA, Kelleher AD, Lloyd A, Lewin SR.

Clin Vaccine Immunol. 2007 Jan;14(1):52-9. Epub 2006 Oct 18.PMID: 17050740 [PubMed - indexed for MEDLINE]Related articlesFree article

Optimal preparation of rhesus macaque blood for cytokine flow cytometric analysis.

Nomura LE, deHaro ED, Martin LN, Maecker HT.

Cytometry A. 2003 May;53(1):28-38.PMID: 12701130 [PubMed - indexed for MEDLINE]Related articlesFree article

Simultaneous activation of viral antigen-specific memory CD4+ and CD8+ T-cells using mRNA-electroporated CD40-activated autologous B-cells.

Van den Bosch GA, Van Gulck E, Ponsaerts P, Nijs G, Lenjou M, Apers L, Kint I, Heyndrickx L, Vanham G, Van Bockstaele DR, Berneman ZN, Van Tendeloo VF.

J Immunother. 2006 Sep-Oct;29(5):512-23.PMID: 16971807 [PubMed - indexed for MEDLINE]Related articles

Antigen-specific T cell responses induced by Towne cytomegalovirus (CMV) vaccine in CMV-seronegative vaccine recipients.

Jacobson MA, Sinclair E, Bredt B, Agrillo L, Black D, Epling CL, Carvidi A, Ho T, Bains R, Adler SP.

J Clin Virol. 2006 Mar;35(3):332-7. Epub 2006 Jan 18.PMID: 16387547 [PubMed - indexed for MEDLINE]Related articles

Evaluation of a novel mononuclear cell isolation procedure for serological HLA typing.

Schlenke P, Klüter H, Müller-Steinhardt M, Hammers HJ, Borchert K, Bein G.

Clin Diagn Lab Immunol. 1998 Nov;5(6):808-13.PMID: 9801339 [PubMed - indexed for MEDLINE]Related articlesFree article

Multifunctional human immunodeficiency virus (HIV) gag-specific CD8+ T-cell responses in rectal mucosa and peripheral blood mononuclear cells during chronic HIV type 1 infection.

Critchfield JW, Lemongello D, Walker DH, Garcia JC, Asmuth DM, Pollard RB, Shacklett BL.

J Virol. 2007 Jun;81(11):5460-71. Epub 2007 Mar 7.PMID: 17344302 [PubMed - indexed for MEDLINE]Related articlesFree article

Viability and recovery of peripheral blood mononuclear cells cryopreserved for up to 12 years in a multicenter study.

Kleeberger CA, Lyles RH, Margolick JB, Rinaldo CR, Phair JP, Giorgi JV.

Clin Diagn Lab Immunol. 1999 Jan;6(1):14-9.PMID: 9874657 [PubMed - indexed for MEDLINE]Related articlesFree article

Effect of cryopreservation on measurement of cytomegalovirus-specific cellular immune responses in HIV-infected patients.

Weinberg A, Wohl DA, Brown DG, Pott GB, Zhang L, Ray MG, van der Horst C.

J Acquir Immune Defic Syndr. 2000 Oct 1;25(2):109-14.PMID: 11103040 [PubMed - indexed for MEDLINE]Related articles

Rapid freezing of whole blood or buffy coat samples for polymerase chain reaction and cell culture analysis: application to detection of human immunodeficiency virus in blood donor and recipient repositories. The Transfusion Safety Study Group.

Adams M, Lee TH, Busch MP, Heitman J, Marshall GJ, Gjerset GF, Mosley JW.

Transfusion. 1993 Jun;33(6):504-8.PMID: 8516793 [PubMed - indexed for MEDLINE]Related articles

Effects of specimen collection, processing, and storage conditions on stability of human immunodeficiency virus type 1 RNA levels in plasma.

Ginocchio CC, Wang XP, Kaplan MH, Mulligan G, Witt D, Romano JW, Cronin M, Carroll R.

J Clin Microbiol. 1997 Nov;35(11):2886-93.PMID: 9350753 [PubMed - indexed for MEDLINE]Related articlesFree article

DNAse treatment following thawing of cryopreserved PBMC is a procedure suitable for lymphocyte functional studies.

García-Piñeres AJ, Hildesheim A, Williams M, Trivett M, Strobl S, Pinto LA.

J Immunol Methods. 2006 Jun 30;313(1-2):209-13. Epub 2006 May 17.PMID: 16737707 [PubMed - indexed for MEDLINE]Related articles