PubMed

Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C.

Ailenberg M, Goldenberg NM, Silverman M.

BMC Biotechnol. 2005 Sep 1;5:23.PMID: 16137330 [PubMed - indexed for MEDLINE]Related articlesFree article

SORS: a universal one-round PCR-based method for site-directed mutagenesis.

Wang BL, Quan JX, Liang H, Jiao YL, Li XX, Guo G, Zhang JY.

Mol Biotechnol. 2008 Sep;40(1):69-75. Epub 2008 Apr 16.PMID: 18415714 [PubMed - indexed for MEDLINE]Related articles

Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis.

Stemmer WP, Morris SK.

Biotechniques. 1992 Aug;13(2):214-20.PMID: 1327007 [PubMed - indexed for MEDLINE]Related articles

Precise large deletions by the PCR-based overlap extension method.

Senanayake SD, Brian DA.

Mol Biotechnol. 1995 Aug;4(1):13-5.PMID: 8521036 [PubMed - indexed for MEDLINE]Related articles

Site-directed mutagenesis by double polymerase chain reaction.

Barik S.

Mol Biotechnol. 1995 Feb;3(1):1-7.PMID: 7606501 [PubMed - indexed for MEDLINE]Related articles

A novel method for site-directed mutagenesis using PCR and uracil DNA glycosylase.

Rashtchian A, Thornton CG, Heidecker G.

PCR Methods Appl. 1992 Nov;2(2):124-30.PMID: 1477668 [PubMed - indexed for MEDLINE]Related articlesFree article

A novel simple and rapid PCR-based site-directed mutagenesis method.

Rabhi I, Guedel N, Chouk I, Zerria K, Barbouche MR, Dellagi K, Fathallah DM.

Mol Biotechnol. 2004 Jan;26(1):27-34.PMID: 14734821 [PubMed - indexed for MEDLINE]Related articles

Gene splicing and mutagenesis by PCR-driven overlap extension.

Heckman KL, Pease LR.

Nat Protoc. 2007;2(4):924-32.PMID: 17446874 [PubMed - indexed for MEDLINE]Related articles

PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes.

Horton RM.

Mol Biotechnol. 1995 Apr;3(2):93-9.PMID: 7620981 [PubMed - indexed for MEDLINE]Related articles

Description of a one step staggered reannealing method for directional cloning of PCR-generated DNA using sticky-end ligation without employing restriction enzymes.

Ailenberg M, Silverman M.

Biochem Mol Biol Int. 1996 Jul;39(4):771-9.PMID: 8843346 [PubMed - indexed for MEDLINE]Related articles

Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer.

Kato Y, Arakawa N, Masuishi Y, Kawasaki H, Hirano H.

J Biosci Bioeng. 2009 Jan;107(1):95-7.PMID: 19147118 [PubMed - indexed for MEDLINE]Related articles

Deletion mutagenesis of large areas in Plasmodium falciparum genes: a comparative study.

Williams M, Louw AI, Birkholtz LM.

Malar J. 2007 May 22;6:64.PMID: 17519001 [PubMed - indexed for MEDLINE]Related articlesFree article

[An improved PCR-based megaprimer method for site-directed mutagenesis]

Mo Q, Xu X, Zhong X, Liu Z.

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002 Feb;19(1):68-71. Chinese. PMID: 11836693 [PubMed - indexed for MEDLINE]Related articles

Splicing together different regions of a gene by modified polymerase chain reaction-based site-directed mutagenesis.

Li X, Qiu Y, Shen Y, Ding C, Liu P, Zhou J, Ma Z.

Anal Biochem. 2008 Feb 15;373(2):398-400. Epub 2007 Oct 23.PMID: 18022376 [PubMed - indexed for MEDLINE]Related articles

Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization.

Young DD, Lusic H, Lively MO, Deiters A.

Nucleic Acids Res. 2009 May;37(8):e58. Epub 2009 Mar 17.PMID: 19293272 [PubMed - indexed for MEDLINE]Related articlesFree article

Novel methods for cloning and engineering genes using the polymerase chain reaction.

Rashtchian A.

Curr Opin Biotechnol. 1995 Feb;6(1):30-6. Review.PMID: 7894080 [PubMed - indexed for MEDLINE]Related articles

PCR induction of a TaqI restriction site at any CpG dinucleotide using two mismatched primers (CpG-PCR).

O'Dell SD, Humphries SE, Day IN.

Genome Res. 1996 Jun;6(6):558-68.PMID: 8828045 [PubMed - indexed for MEDLINE]Related articlesFree article

Random mutagenesis by whole-plasmid PCR amplification.

Parikh A, Guengerich FP.

Biotechniques. 1998 Mar;24(3):428-31.PMID: 9526653 [PubMed - indexed for MEDLINE]Related articles

A method for clone sequence confirmation using a mismatch-specific DNA endonuclease.

Qiu P, Shandilya H, Gerard GF.

Mol Biotechnol. 2005 Jan;29(1):11-8.PMID: 15668515 [PubMed - indexed for MEDLINE]Related articles

Directed mutagenesis using the polymerase chain reaction.

Cormack B.

Curr Protoc Neurosci. 2001 May;Chapter 4:Unit 4.11.PMID: 18428475 [PubMed - indexed for MEDLINE]Related articles