PubMed

A step ahead: combining protein purification and correct folding selection.

de Marco A.

Microb Cell Fact. 2004 Oct 7;3(1):12.PMID: 15471550 [PubMed - as supplied by publisher]Related articlesFree article

Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains.

Olichon A, Schweizer D, Muyldermans S, de Marco A.

BMC Biotechnol. 2007 Jan 26;7:7.PMID: 17257422 [PubMed - indexed for MEDLINE]Related articlesFree article

Synthesis of degenerated libraries of the ras-binding domain of raf and rapid selection of fast-folding and stable clones with the dihydrofolate reductase protein fragment complementation assay.

Campbell-Valois FX, Michnick SW.

Methods Mol Biol. 2007;352:249-74.PMID: 17041270 [PubMed - indexed for MEDLINE]Related articles

Simplified characterization through site-specific protease-mediated release of affinity proteins selected by staphylococcal display.

Kronqvist N, Löfblom J, Severa D, Ståhl S, Wernérus H.

FEMS Microbiol Lett. 2008 Jan;278(1):128-36. Epub 2007 Nov 21.PMID: 18034830 [PubMed - indexed for MEDLINE]Related articles

Induced fit of passenger proteins fused to Archaea maltose binding proteins.

Huang H, Liu J, de Marco A.

Biochem Biophys Res Commun. 2006 May 26;344(1):25-9. Epub 2006 Mar 31.PMID: 16615999 [PubMed - indexed for MEDLINE]Related articles

Purification of polyclonal anti-conformational antibodies for use in affinity selection from random peptide phage display libraries: a study using the hydatid vaccine EG95.

Read AJ, Gauci CG, Lightowlers MW.

J Chromatogr B Analyt Technol Biomed Life Sci. 2009 May 15;877(14-15):1516-22. Epub 2009 Mar 28.PMID: 19349218 [PubMed - indexed for MEDLINE]Related articlesFree article

Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display.

Steiner D, Forrer P, Plückthun A.

J Mol Biol. 2008 Oct 24;382(5):1211-27. Epub 2008 Aug 6.PMID: 18706916 [PubMed - indexed for MEDLINE]Related articles

A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production.

Cabrita LD, Dai W, Bottomley SP.

BMC Biotechnol. 2006 Mar 1;6:12.PMID: 16509985 [PubMed - indexed for MEDLINE]Related articlesFree article

Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.

de Marco A.

Nat Protoc. 2007;2(10):2632-9.PMID: 17948006 [PubMed - indexed for MEDLINE]Related articles

Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors.

Dümmler A, Lawrence AM, de Marco A.

Microb Cell Fact. 2005 Dec 13;4:34.PMID: 16351710 [PubMed]Related articlesFree article

Small-scale, semi-automated purification of eukaryotic proteins for structure determination.

Frederick RO, Bergeman L, Blommel PG, Bailey LJ, McCoy JG, Song J, Meske L, Bingman CA, Riters M, Dillon NA, Kunert J, Yoon JW, Lim A, Cassidy M, Bunge J, Aceti DJ, Primm JG, Markley JL, Phillips GN Jr, Fox BG.

J Struct Funct Genomics. 2007 Dec;8(4):153-66. Epub 2007 Nov 6.PMID: 17985212 [PubMed - indexed for MEDLINE]Related articlesFree article

Influence of the protein oligomericity on final yield after affinity tag removal in purification of recombinant proteins.

Kenig M, Peternel S, Gaberc-Porekar V, Menart V.

J Chromatogr A. 2006 Jan 6;1101(1-2):293-306. Epub 2005 Oct 26.PMID: 16256128 [PubMed - indexed for MEDLINE]Related articles

Chaperone over-expression in Escherichia coli: apparent increased yields of soluble recombinant protein kinases are due mainly to soluble aggregates.

Haacke A, Fendrich G, Ramage P, Geiser M.

Protein Expr Purif. 2009 Apr;64(2):185-93. Epub 2008 Nov 11.PMID: 19038347 [PubMed - indexed for MEDLINE]Related articles

Rapid isolation of single-chain antibodies for structural genomics.

Shea C, Bloedorn L, Sullivan MA.

J Struct Funct Genomics. 2005;6(2-3):171-5.PMID: 16211515 [PubMed - indexed for MEDLINE]Related articles

One-step affinity purification of recombinant urokinase-type plasminogen activator receptor using a synthetic peptide developed by combinatorial chemistry.

Jacobsen B, Gårdsvoll H, Juhl Funch G, Ostergaard S, Barkholt V, Ploug M.

Protein Expr Purif. 2007 Apr;52(2):286-96. Epub 2006 Aug 30.PMID: 17027282 [PubMed - indexed for MEDLINE]Related articles

Strategies for folding of affinity tagged proteins using GroEL and osmolytes.

Katayama H, McGill M, Kearns A, Brzozowski M, Degner N, Harnett B, Kornilayev B, Matković-Calogović D, Holyoak T, Calvet JP, Gogol EP, Seed J, Fisher MT.

J Struct Funct Genomics. 2009 Mar;10(1):57-66. Epub 2008 Dec 12.PMID: 19082872 [PubMed - indexed for MEDLINE]Related articles

Generation of monoclonal antibodies for the assessment of protein purification by recombinant ribosomal coupling.

Kristensen J, Sperling-Petersen HU, Mortensen KK, Sørensen HP.

Int J Biol Macromol. 2005 Dec 15;37(4):212-7. Epub 2005 Dec 2.PMID: 16330094 [PubMed - indexed for MEDLINE]Related articles

A set of ligation-independent in vitro translation vectors for eukaryotic protein production.

Bardóczy V, Géczi V, Sawasaki T, Endo Y, Mészáros T.

BMC Biotechnol. 2008 Mar 27;8:32.PMID: 18371187 [PubMed - indexed for MEDLINE]Related articlesFree article

Folding and purification of insoluble (inclusion body) proteins from Escherichia coli.

Wingfield PT, Palmer I, Liang SM.

Curr Protoc Protein Sci. 2001 May;Chapter 6:Unit 6.5.PMID: 18429192 [PubMed - indexed for MEDLINE]Related articles

Affinity purification of insoluble recombinant fusion proteins containing glutathione-S-transferase.

Hartman J, Daram P, Frizzell RA, Rado T, Benos DJ, Sorscher EJ.

Biotechnol Bioeng. 1992 Apr 5;39(8):828-32.PMID: 18601017 [PubMed]Related articles