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    Plant Physiol. 1999 Feb;119(2):507-10.

    Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.

    Helliwell CA, Poole A, Peacock WJ, Dennis ES.

    Commonwealth Scientific and Industrial Research Organization, Plant Industry, G.P.O. Box 1600, Canberra, ACT 2601, Australia. chrish@pican.pi.csiro.au

    The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ent-kaurene to ent-kaurenoic acid.

    PMID: 9952446 [PubMed - indexed for MEDLINE]

    PMCID: 32127

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