Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine.
The polypeptide sequence of the unmodified catalase from Penicillium vitale containing 696 amino acid residues was deduced. The sequences of 76 tryptic peptides of the unmodified catalase, 63 tryptic peptides of the catalase with modified Lys residues, 48 peptides resulting from catalase cleavage by the Staphylococcus aureus V8 protease, and 9 fragments obtained by BrCN-treatment were considered, and a comparison with the sequences of other catalases was made.