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    Biochim Biophys Acta. 1998 Jan 21;1395(2):237-45.

    Cloning and sequencing of human betaIII-tubulin cDNA: induction of betaIII isotype in human prostate carcinoma cells by acute exposure to antimicrotubule agents.

    Ranganathan S, Dexter DW, Benetatos CA, Hudes GR.

    Department of Medicine, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.

    Antimicrotubule drugs are used as chemotherapeutic agents due to their effects on essential cellular functions such as mitosis, organelle transport and maintenance of cell shape. When used in combination, paclitaxel with estramustine or vinblastine has demonstrated activity against hormone refractory prostate cancer. To understand the mechanism of resistance that develops in patients as a result of antimicrotubule drug therapy, we exposed human prostate carcinoma cells to IC20 and IC40 doses of estramustine, paclitaxel or vinblastine for 48 h and examined the beta-tubulin (the cellular target) isotype composition. The results revealed an increase in the betaIII-tubulin isotype as a result of drug treatment both at protein and message levels. In addition, examination of human brain cell lines with different intrinsic levels of betaIII showed that cell lines with higher betaIII levels were more resistant to paclitaxel. These results are in agreement with our previous findings in human prostate carcinoma cell lines that were made resistant to estramustine or paclitaxel and suggest an important function for betaIII in antimicrotubule drug resistance. Also, the complete coding sequence of human betaIII tubulin reported here will provide molecular tools for future investigations.

    PMID: 9473684 [PubMed - indexed for MEDLINE]

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    Patient drug information

    • Vinblastine (Velban®)

      Your doctor has ordered the drug vinblastine to help treat your illness. The drug is given by injection into a vein.

    • Estramustine (Emcyt®)

      Estramustine is used to treat prostate cancer that has worsened or has spread to other parts of the body. Estramustine is in a class of medications called antimicrotubule agents. It works by stopping the growth and sprea...

    • Source: AHFS Consumer Medication Information

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