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    Nat Struct Biol. 1997 Aug;4(8):618-22.

    Trapping and visualization of a covalent enzyme-phosphate intermediate.

    Murphy JE, Stec B, Ma L, Kantrowitz ER.

    Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.

    PMID: 9253408 [PubMed - indexed for MEDLINE]

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