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    Biochim Biophys Acta. 1997 Jun 26;1352(3):233-7.

    Molecular cloning and sequencing of the cDNA encoding the catalytic subunit of mouse glutamate-cysteine ligase.

    Reid LL, Botta D, Lu Y, Gallagher EP, Kavanagh TJ.

    Department of Environmental Health, University of Washington, Seattle, USA.

    Reverse transcription-polymerase chain reaction (RT-PCR) was used for the enzymatic synthesis of cDNA sequences encompassing the open reading frame for the catalytic subunit of mouse kidney glutamate-cysteine ligase (Glclc). Comparison of the mouse Glclc cDNA sequence and predicted protein sequence with that of rat Glclc and human GLCLC revealed between 94.8% and 88.4% cDNA homology and 98.4% to 95% amino acid identity, respectively.

    PMID: 9224945 [PubMed - indexed for MEDLINE]

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