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    Brain Res Mol Brain Res. 1996 Nov;42(1):25-30.

    Molecular cloning of chick pineal tryptophan hydroxylase and circadian oscillation of its mRNA levels.

    Florez JC, Seidenman KJ, Barrett RK, Sangoram AM, Takahashi JS.

    Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208-3520, USA.

    We have previously shown that the level of [35S]methionine incorporation into tryptophan hydroxylase (TPH) shows a circadian rhythm in cultured chick pineal cells. The TPH protein oscillation persists in constant darkness, peaks in the early night and can be phase-shifted by light, in parallel to the effect of these treatments on melatonin synthesis. We have cloned and sequenced a full-length cDNA for chick pineal TPH. Levels of TPH mRNA show a robust diurnal oscillation both in vivo and in vitro. The rhythm in TPH mRNA also persists in constant darkness, suggesting that TPH mRNA synthesis and/or turnover is regulated by an endogenous circadian clock in cultured chick pineal cells. The circadian oscillation of TPH constitutes the first described circadian rhythm of a chick pineal gene at the mRNA level.

    PMID: 8915576 [PubMed - indexed for MEDLINE]

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