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    Gene. 1996 Jun 26;172(2):207-9.

    Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua.

    Matsushita Y, Kang W, Charlwood BV.

    Division of Life Sciences, King's College London, UK.

    A cDNA encoding farnesyl diphosphate (FPP) synthase (FPPS) has been cloned from a cDNA library of Artemisia annua. The sequence analysis showed that the cDNA encoded a protein of 343 amino acid (aa) residues with a calculated molecular weight of 39420 kDa. The deduced aa sequence of the cDNA was highly similar to FPPS from other plants, yeast and mammals, and contained the two conserved domains found in polyprenyl synthases including FPPS, geranylgeranyl diphosphate synthases and hexaprenyl diphosphate synthases. The expression of the cDNA in Escherichia coli showed enzyme activity for FPPS in vitro.

    PMID: 8682304 [PubMed - indexed for MEDLINE]

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