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    Gene. 1995 Dec 29;167(1-2):25-31.

    Cloning of the mouse gene encoding plasma glutathione peroxidase: organization, sequence and chromosomal localization.

    Schwaab V, Baud E, Ghyselinck N, Mattei MG, Dufaure JP, Drevet JR.

    Laboratoire de Biologie Cellulaire, CNRS, URA1940, Université Blaise Pascal, Aubière, France.

    Using a reverse transcription coupled to PCR amplification strategy, with degenerated primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, we have generated, from mouse epididymal RNA, a cDNA fragment which was subsequently used to isolate a genomic clone encoding mouse plasma GPX (GPX3). GPX3 is a major enzyme in reducing lipid hydroperoxides and hydrogen peroxide in plasma. We confirm here that the mouse epididymis is a new site of expression of GPX3 and report, together with the sequence, the structural analysis and the chromosomal localization of the mouse GPX3 single-copy gene to chromosome 11.

    PMID: 8566787 [PubMed - indexed for MEDLINE]

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