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    Gene. 1993 Aug 25;130(2):271-5.

    Characterization of the pyruvate kinase-encoding gene (pki1) of Trichoderma reesei.

    Schindler M, Mach RL, Vollenhofer SK, Hodits R, Gruber F, Visser J, De Graaff L, Kubicek CP.

    Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Vienna, Austria.

    The pyruvate kinase-encoding gene (pki1) from Trichoderma reesei was isolated by hybridization to the corresponding Aspergillus nidulans pkiA gene. The 1614-bp nucleotide (nt) sequence of the cloned gene codes for a 538-amino-acid protein. The coding sequence contains a single intron of 246 nt at a position identical to that of intron E in the A. nidulans gene. The PKI protein shows extensive homology to the PKIs of A. nidulans and A. niger (67%) and Saccharomyces cerevisiae (59%). The 5' non-coding sequence contains a number of motifs typical for yeast glycolytic genes, but so far only rarely found in filamentous fungi.

    PMID: 8359694 [PubMed - indexed for MEDLINE]

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