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    Biochim Biophys Acta. 1995 Jan 2;1260(1):109-12.

    Cloning and expression analysis of a cytochrome P-450(11 beta) cDNA in sheep.

    Boon WC, Roche PJ, Hammond VE, Jeyaseelan K, Crawford RJ, Coghlan JP.

    Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.

    A full length ovine steroid 11 beta-hydroxylase (cytochrome P-450(11 beta)) cDNA clone from a sheep adrenal cortex cDNA library was isolated. Sequence analysis indicates that this cDNA clone resembles bovine P-450(11 beta) cDNA (95% nucleotide sequence homology) more closely than rat P-450(11 beta) cDNA (69% nucleotide sequence homology). Although the levels of nucleotide sequence homology of this cDNA clone to the rat P-450(11 beta) cDNA and the rat P-450aldo cDNA are similar, the putative amino acid sequence shows a closer resemblance to rat P-450aldo protein. Northern blot analysis shows that there are three sizes of transcript and they are expressed throughout the adrenal cortex.

    PMID: 7999785 [PubMed - indexed for MEDLINE]

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