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    FEMS Microbiol Lett. 1995 Jan 15;125(2-3):219-24.

    Distribution of the flavohaemoglobin, HMP, between periplasm and cytoplasm in Escherichia coli.

    Vasudevan SG, Tang P, Dixon NE, Poole RK.

    Department of Molecular Sciences, James Cook University, Townsville, Australia.

    The subcellular distribution of the soluble flavohaemoglobin (HMP) of Escherichia coli has been determined. Cells over-expressing HMP from the cloned hmp gene on a multicopy plasmid were fractionated by osmotic shock and lysozyme treatment. Spectral analysis of subcellular fractions showed the CO-binding haemoprotein to be cytoplasmic. However, Western blotting using antibody raised to purified HMP revealed approximately 30% of the protein to be periplasmic in the over-expressing strain. Western analysis also revealed substantial levels of periplasmic HMP in a strain expressing only chromosomally encoded protein but none in an hmp mutant. The results are discussed in relation to protein function and the similar distribution reported for Vitreoscilla globin.

    PMID: 7875569 [PubMed - indexed for MEDLINE]

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