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    Biochem Mol Biol Int. 1994 Sep;34(2):309-13.

    Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone.

    Umekawa T, Kurita T.

    Department of Urology, Kinki University School of Medicine, Osaka, Japan.

    Soluble organic matrix was extracted with 0.1 M EDTA from calcium oxalate monohydrate urinary stone (CaOx), purified by SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes for the NH2-terminal amino acid sequence analysis. A protein at 30 kDa showed complete homology with calprotectin (20 amino acids in the NH2-terminal), so we termed this a calprotectin-like protein. Calprotectin which was extracted from human granulocytes had inhibitory activity in calcium oxalate monohydrate crystal growth in vitro. This study suggested that, calprotectin-like protein detected in CaOx is thought to be a potent inhibitor of crystal growth and may therefore be important in the etiology of CaOx formation.

    PMID: 7849642 [PubMed - indexed for MEDLINE]

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