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    Gene. 1995 Apr 14;156(1):123-7.

    Isolation, characterization and overexpression of the yeast gene, GLR1, encoding glutathione reductase.

    Collinson LP, Dawes IW.

    School of Biochemistry and Molecular Genetics, University of New South Wales, Sydney, Australia.

    Using degenerate oligodeoxyribonucleotides based on the N-terminal amino acid (aa) sequence of a yeast glutathione reductase (GR) CNBr-generated peptide fragment and a conserved C-terminal region of known GR aa sequences, the yeast gene encoding GR, GLR1, was isolated using PCR followed by screening of a yeast genomic DNA plasmid library. GLR1 encodes a 467-aa protein with a deduced M(r) of 51,545. Comparison with Escherichia coli and human GR sequences reveals 49.8% aa identity. Yeast cells transformed with a multicopy plasmid containing the genomic clone overproduced GR activity sixfold. GLR1 was found not to be an essential gene.

    PMID: 7737505 [PubMed - indexed for MEDLINE]

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