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    Proc Natl Acad Sci U S A. 1983 Jan;80(2):349-52.

    Isolation of cDNA sequences coding for a part of human tissue plasminogen activator.

    Edlund T, Ny T, Rånby M, Hedén LO, Palm G, Holmgren E, Josephson S.

    We have isolated a cDNA sequence coding for a part of human tissue plasminogen activator. mRNA coding for tissue plasminogen activator was partially purified, copied into double-stranded cDNA, and cloned into Escherichia coli. Two sets of partially overlapping oligodeoxynucleotide mixtures corresponding to all possible coding sequences for a known portion of the tissue plasminogen activator gene were prepared. One set was used as a probe to screen cDNA containing bacterial clones and both were used as probes in hybridization against purified plasmid DNA. Of 4,200 bacterial clones examined, 1 carried a plasmid that hybridized to both sets of oligonucleotides. This plasmid contained a 370-base-pair cDNA insert, which was shown by nucleotide sequence analysis to code for the cleavage site region in the one-chain form of the human tissue plasminogen activator.

    PMID: 6572897 [PubMed - indexed for MEDLINE]

    PMCID: 393374

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