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    FEBS Lett. 1983 Jun 13;156(2):307-10.

    The TolC protein of Escherichia coli K12 is synthesised in a precursor form.

    Hackett J, Misra R, Reeves P.

    We examined the biosynthesis of the TolC protein of Escherichia coli K12 in a pulse-chase experiment, followed by immunoprecipitation with anti-TolC antibody and SDS-PAGE of the immunoprecipitate. This showed that TolC protein was originally synthesised in a precursor form (Mr 54 500) which could be chased into the mature form (Mr 52 000). DNA sequencing of a portion of the cloned tolC gene showed that the N-terminus of the mature rotein was preceded by a typical signal sequence of 22 residues (Mr 2542). The initiator Met was preceded by a Shine-Dalgarno sequence, with the correct spacing.

    PMID: 6303857 [PubMed - indexed for MEDLINE]

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