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    Nucleic Acids Res. 1984 Dec 11;12(23):9039-49.

    Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR.

    Pósfai G, Baldauf F, Erdei S, Pósfai J, Venetianer P, Kiss A.

    The nucleotide sequence of the gene coding for the 5'-GGCC and 5'-CCGG specific DNA methyltransferase of the Bacillus subtilis phage SPR was determined by the Maxam-Gilbert procedure. Transcriptional and translational signals of the sequence were assigned with the help of S1 mapping and translation in E. coli minicells. The gene codes for a 49 kd polypeptide. The amino acid sequence of the SPR methylase shows regions of homology with the sequence of the 5'-GGCC-specific BspRI modification methylase.

    PMID: 6096817 [PubMed - indexed for MEDLINE]

    PMCID: 320436

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