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    J Bacteriol. 1985 Mar;161(3):1059-68.

    Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli.

    Rule GS, Pratt EA, Chin CC, Wold F, Ho C.

    Recombinant DNA plasmids containing the gene for the membrane-bound D-lactate dehydrogenase (D-LDH) of Escherichia coli linked to the promoter PL from lambda were constructed. After induction, the levels of D-LDH were elevated 300-fold over that of the wild type and amounted to 35% of the total cellular protein. The nucleotide sequence of the D-LDH gene was determined and shown to agree with the amino acid composition and the amino-terminal sequence of the purified enzyme. Removal of the amino-terminal formyl-Met from D-LDH was not inhibited in cells which contained these high levels of D-LDH.

    PMID: 3882663 [PubMed - indexed for MEDLINE]

    PMCID: 215007

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