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    Nucleic Acids Res. 1986 Nov 11;14(21):8595-603.

    Sequencing and identification of a cDNA clone for tomato polygalacturonase.

    Grierson D, Tucker GA, Keen J, Ray J, Bird CR, Schuch W.

    The 2a isoenzyme of tomato polygalacturonase was purified from ripe fruit and characterised. The N-terminal amino acid sequence of the protein was determined in order to identify polygalacturonase cDNA clones. The nucleotide sequence of a ripening-related cDNA (pTOM 6) was determined and found to encode the N-terminal sequence of mature polygalacturonase 2a. The complete open reading frame encodes a polypeptide of molecular weight 50,051, including a putative pre-sequence of 71 amino acids.

    PMID: 3786135 [PubMed - indexed for MEDLINE]

    PMCID: 311879

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