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    J Biochem. 1988 Nov;104(5):683-6.

    Expression of two kinds of cytochrome P-450(11 beta) mRNA in bovine adrenal cortex.

    Kirita S, Morohashi K, Hashimoto T, Yoshioka H, Fujii-Kuriyama Y, Omura T.

    Department of Biology, Faculty of Science, Kyushu University, Fukuoka.

    Using pcP-450(11 beta)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11 beta)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11 beta)-3 was highly homologous but not identical with that of pcP-450(11 beta)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11 beta)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11 beta) mRNA were expressed in individual animals and that at least two kinds of P-450(11 beta) genes exist in the bovine genome.

    PMID: 3266212 [PubMed - indexed for MEDLINE]

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