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    Gene. 1989 Jul 15;79(2):355-60.

    Cloning, expression and sequence homologies of cDNA for human gamma enolase.

    Oliva D, Barba G, Barbieri G, Giallongo A, Feo S.

    Istituto di Biologia dello Sviluppo (Consiglio Nazionale delle Ricerche), Palermo, Italy.

    The nucleotide sequence of the human gamma-enolase mRNA was determined from recombinant cDNA clones. The sequence spans 2273 bp and includes the complete coding region of 1299 bp, a 5'-noncoding region of 74 bp and a 897-bp-long 3'-noncoding region containing a variant polyadenylation signal (ATTAAA). The deduced amino acid (aa) sequence is 433 aa long and shows a 97% similarity with rat gamma-enolase. Both the 5'- and 3'-untranslated regions are similar (82% and 68%, respectively) to the analogous regions of the rat gamma-enolase gene, suggesting that a strong selective pressure operates on noncoding segments of gamma-enolase mRNAs. The size of the gamma-enolase mRNA expressed in human brain is 2.4 kb. A crosshybridizing 1.5-kb message is detected in human skeletal muscle which may be derived from the beta-enolase-coding gene.

    PMID: 2792767 [PubMed - indexed for MEDLINE]

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