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    FEBS Lett. 1989 Sep 11;255(1):63-6.

    Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3.

    Kriechbaum M, Heilmann HJ, Wientjes FJ, Hahn M, Jany KD, Gassen HG, Sharif F, Alaeddinoglu G.

    Institut für Biochemie, Technische Hochschule Darmstadt, FRG.

    A cDNA library from Aspergillus niger strain NRRL-3 enriched in sequences glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12,000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5'- and a longer 3'-noncoding region. The deduced protein sequence was verified by partial peptide sequencing.

    PMID: 2792372 [PubMed - indexed for MEDLINE]

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