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    Immunol Lett. 1989 Dec;23(2):133-7.

    A single-step purification procedure and partial amino acid sequence analysis of picomole amounts of the rat T cell alloantigen RT6.2.

    Kashan A, Buck F, Haag F, Koch F, Thiele HG.

    Dept. of Immunology, University Hospital, Hamburg, F.R.G.

    A single-step immunoaffinity purification procedure for the rat T cell marker RT6.2 is described which permits the isolation of microgram quantities of protein from the RT6.2+ T-T hybridoma EpD3. The amino terminus was sequenced directly from a polyvinylidene (PVDF) membrane blot prepared after SDS-PAGE. Further internal sequence data were obtained from peptides generated from purified RT6.2 digested with different endoproteases and separated by reverse-phase micro-HPLC. A computer search in data banks did not reveal any significant homology to other proteins.

    PMID: 2632369 [PubMed - indexed for MEDLINE]

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