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    J Bacteriol. 1989 Jan;171(1):577-80.

    Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene.

    Matsuyama A, Yamamoto H, Nakano E.

    Research and Development Division, Kikkoman Corporation, Chiba-ken, Japan.

    The Escherichia coli K-12 ackA gene, which encodes an acetate kinase, was cloned. The acetate kinase activities of ackA+ plasmid-containing strains were amplified 160- to 180-fold. The complete nucleotide sequence of the ackA gene was determined. It was deduced that the ackA gene coded for a protein of 400 amino acids with an Mr of 43,297. The ackA gene was found to be located about 15 kilobases upstream of the purF-folC-hisT region of the chromosome.

    PMID: 2536666 [PubMed - indexed for MEDLINE]

    PMCID: 209626

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