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    Mol Endocrinol. 1987 Apr;1(4):327-31.

    Cloning and nucleotide sequence analysis of complementary deoxyribonucleic acid for bovine preproinsulin.

    D'Agostino J, Younes MA, White JW, Besch PK, Field JB, Frazier ML.

    Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas 77030.

    A cDNA library was prepared from poly(A+) RNA isolated from fetal bovine pancreas. Bacterial colonies were screened for sequences homologous to a rat preproinsulin I cDNA probe. Ten positive clones were selected at random and further studied. Northern blot analyses revealed that seven of these clones hybridized to a single RNA species, of approximately 400 nucleotides. Sequence analysis of one of these clones (pbI2885) revealed the entire structural region of bovine preproinsulin mRNA including a 72 nucleotide region encoding a signal peptide enriched in hydrophobic residues. The overall nucleotide homology between bovine and human preproinsulin mRNA was 76% for the preregion, 89% for the A chain, 83% for the B chain, and 68% for the C peptide (including a 15 nucleotide deletion).

    PMID: 2456452 [PubMed - indexed for MEDLINE]

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