Department of Medical Biochemistry, University of Galgary, Canada.
A cDNA (TCK1) encoding a creatine kinase (CK) subunit has been isolated from a cDNA library prepared from rainbow trout testis poly(A) + RNA. The predicted amino acid sequence for this CK subunit showed a novel amino-terminal coding region. Northern blot analyses demonstrated preferential expression of this CK subunit in testis relative to other CK containing organs. Southern blot analysis suggested a single copy number for this CK gene.