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    Biochem Biophys Res Commun. 1991 Jan 15;174(1):142-8.

    Isolation and identification of C-type natriuretic peptide in chicken brain.

    Arimura JJ, Minamino N, Kangawa K, Matsuo H.

    Department of Biochemistry, Miyazaki Medical College, Japan.

    C-type natriuretic peptide (CNP) has recently been identified in porcine brain as a third member of the mammalian natriuretic peptide family (1). Using a radioimmunoassay system for porcine CNP, we found a significant concentration of immunoreactive (ir-) CNP in chicken brain, from which a new peptide was isolated. By microsequence analysis, the amino acid sequence of the peptide was determined to be Gly-Leu-Ser-Arg-Ser-Cys-Phe- Gly-Val-Lys-Leu-Asp-Arg-Ile-Gly-Ser-Met-Ser-Gly-Leu-Gly-Cys. Based on its high homology to porcine CNP, the peptide was designated chicken C-type natriuretic peptide. Chicken CNP also elicits pharmacological effects highly similar to porcine CNP, suggesting that CNP functions as a neuropeptide in the chicken central nervous system.

    PMID: 1989595 [PubMed - indexed for MEDLINE]

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