My NCBI | Sign In
RSS Settings
  • Search:

Display Settings:

Format

Send to:

Choose Destination

    J Bacteriol. 1991 Dec;173(23):7705-10.

    Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase.

    Gosalbes MJ, Pérez-González JA, González R, Navarro A.

    Asociación de Investigación de Cerveza y Matal, Valencia, Spain.

    Two genes, xynD and gluB, encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase (lichenase) from Bacillus polymyxa have been cloned and expressed in Escherichia coli and Bacillus subtilis. A sequenced DNA fragment of 4,466 bp contains both genes, which are separated by 155 bp. The xynD and gluB genes encode proteins of 67.8 kDa (XYND) and 27 kDa (GLUB). Two peptides with molecular masses of 62 and 53 kDa appear in cell extracts of E. coli and culture supernatants of B. subtilis clones containing the xynD gene. Both peptides show xylanase activity in zymogram analysis. The XYND enzyme also shows alpha-L-arabinofuranosidase activity. The XYND peptide and the xylanase XYNZ from Clostridium thermocellum (O. Grépinet, M. C. Chebrou, and P. Béguin, J. Bacteriol. 170:4582-4588, 1988) show 64% homology in a stretch of about 280 amino acids.

    PMID: 1938968 [PubMed - indexed for MEDLINE]

    PMCID: 212542

    Publication Types, MeSH Terms, Substances, Secondary Source ID

    Publication Types:

    MeSH Terms:

    Substances:

    Secondary Source ID:

    LinkOut - more resources

    Full Text Sources:

    Research Materials:

    Supplemental Content

    Click here to read Click here to read

    Related articles

    Cited by 16 PubMed Central articles

    All links from this record

    • Related Articles

      Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.

    • Nucleotide

      Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.

    • References for this PMC Article

      Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.

    • Taxonomy via GenBank

      Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).

    • Protein

      Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.

    • Genome

      Genome records that include the current articles as references. These are typically the articles that report the sequencing and analysis of the genome.

    • Free in PMC

      Free full text articles in PMC

    • Cited in PMC

      Full-text articles in the PubMed Central Database that cite the current articles.

    Recent activity