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    FEBS Lett. 1991 Jun 3;283(2):215-8.

    Molecular cloning and sequence analysis of cDNA encoding delta 4-3-ketosteroid 5 beta-reductase of rat liver.

    Onishi Y, Noshiro M, Shimosato T, Okuda K.

    Department of Oral and Maxillofacial Surgery II, Hiroshima University School of Dentistry, Japan.

    A cDNA clone encoding delta 4-3-ketosteroid 5 beta-reductase was isolated from rat liver cDNA libraries using antibodies specific for the enzyme and oligonucleotides as probes. The cDNA contained 981-base pair open reading frame encoding 327 amino acid residues (Mr 37,376) and an unusually long 3'-untranslated region rich in AT sequence in the total length of 3189 base pairs. The predicted amino acid sequence contains the sequences similar to the putative NADPH- and steroid-binding regions.

    PMID: 1710579 [PubMed - indexed for MEDLINE]

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