Gene. 1991 May 15;101(1):89-96.

Cloning and characterisation of the Saccharomyces cerevisiae glycerol-3-phosphate dehydrogenase (GUT2) promoter.

Sleep D, Ogden JE, Roberts NA, Goodey AR.

Delta Biotechnology Limited, Nottingham, U.K.

The Saccharomyces cerevisiae glycerol-3-phosphate dehydrogenase (GUT2) promoter and part of the protein-coding region have been isolated on a 6.3-kb genomic DNA fragment. Nucleotide sequence analysis shows that the promoter has many structural features in common with yeast glycolytic enzyme promoters. Chromosomal mapping indicates that this genomic fragment is located on chromosome XII. The GUT2 promoter has been used to construct a recombinant human albumin (reHA) secretion vector; yeast transformed with this vector secrete reHA into the culture supernatant.

PMID: 1676389 [PubMed - indexed for MEDLINE]

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GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae.
Yeast. 1993 Oct; 9(10):1121-30.

[Yeast. 1993]
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Mol Microbiol. 1995 Jul; 17(1):95-107.

[Mol Microbiol. 1995]
Carbon source-dependent transcriptional regulation of the mitochondrial glycerol-3-phosphate dehydrogenase gene, GUT2, from Saccharomyces cerevisiae.
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[Can J Microbiol. 2000]
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J Biol Chem. 1993 Jan 5; 268(1):153-60.

[J Biol Chem. 1993]
A gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) complements an osmosensitive mutant of Saccharomyces cerevisiae.
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[Mol Microbiol. 1993]
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Cited by 5 PubMed Central articles

Modulation of chaperone gene expression in mutagenized Saccharomyces cerevisiae strains developed for recombinant human albumin production results in increased production of multiple heterologous proteins.
Payne T, Finnis C, Evans LR, Mead DJ, Avery SV, Archer DB, Sleep D. Appl Environ Microbiol. 2008 Dec; 74(24):7759-66. Epub 2008 Oct 17.

[Appl Environ Microbiol. 2008]
GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway.
Albertyn J, Hohmann S, Thevelein JM, Prior BA. Mol Cell Biol. 1994 Jun; 14(6):4135-44.

[Mol Cell Biol. 1994]
Identification of potential target genes for Adr1p through characterization of essential nucleotides in UAS1.
Cheng C, Kacherovsky N, Dombek KM, Camier S, Thukral SK, Rhim E, Young ET. Mol Cell Biol. 1994 Jun; 14(6):3842-52.

[Mol Cell Biol. 1994]
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Cloning and characterisation of the Saccharomyces cerevisiae glycerol-3-phosphate dehydrogenase (GUT2) promoter.

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