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    Biochim Biophys Acta. 1992 May 7;1131(1):69-77.

    cDNA cloning and membrane topology of the rabbit gastric H+/K(+)-ATPase alpha-subunit.

    Bamberg K, Mercier F, Reuben MA, Kobayashi Y, Munson KB, Sachs G.

    Department of Physiology, School of Medicine, UCLA.

    We have cloned and sequenced a cDNA for the rabbit gastric proton-potassium pump (H+/K(+)-ATPase) alpha-subunit. The deduced peptide contains 1035 amino acids (Mr 114,201) and shows 97% sequence identity with the respective rat and hog proteins. A monoclonal antibody 146-14 has been shown previously to react with the extracytoplasmic side of the catalytic H+/K(+)-ATPase subunit and here we show that the epitope is in the region between amino acids 855 and 902 (the numbering of the H+/K(+)-ATPase catalytic subunit throughout the paper refers to the rabbit sequence). The localization of this epitope in conjunction with previously observed trypsin cleavage sites in the C-terminal one third of the enzyme and the hydrophobicity plot of the deduced peptide sequence are evidence for a structural model for the alpha-subunit of the H+/K(+)-ATPase which contains at least ten membrane spanning segments, similar to that deduced for the Ca(2+)-ATPase of sarcoplasmic reticulum.

    PMID: 1316171 [PubMed - indexed for MEDLINE]

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