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    DNA Seq. 1992;3(4):245-6.

    Cloning and sequencing of human kappa-casein cDNA.

    Bergström S, Hansson L, Hernell O, Lönnerdal B, Nilsson AK, Strömqvist M.

    Department of Microbiology, University of Umeå, Sweden.

    A cDNA encoding kappa-casein of human milk was cloned and sequenced. The kappa-casein cDNA was isolated from a lambda gt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native kappa-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a kappa-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids.

    PMID: 1296818 [PubMed - indexed for MEDLINE]

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