Science. 2003 Jul 18;301(5631):376-9.
Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice.
Rice Full-Length cDNA Consortium; National Institute of Agrobiological Sciences Rice Full-Length cDNA Project Team, Kikuchi S, Satoh K, Nagata T, Kawagashira N, Doi K, Kishimoto N, Yazaki J, Ishikawa M, Yamada H, Ooka H, Hotta I, Kojima K, Namiki T, Ohneda E, Yahagi W, Suzuki K, Li CJ, Ohtsuki K, Shishiki T; Foundation of Advancement of International Science Genome Sequencing & Analysis Group, Otomo Y, Murakami K, Iida Y, Sugano S, Fujimura T, Suzuki Y, Tsunoda Y, Kurosaki T, Kodama T, Masuda H, Kobayashi M, Xie Q, Lu M, Narikawa R, Sugiyama A, Mizuno K, Yokomizo S, Niikura J, Ikeda R, Ishibiki J, Kawamata M, Yoshimura A, Miura J, Kusumegi T, Oka M, Ryu R, Ueda M, Matsubara K; RIKEN, Kawai J, Carninci P, Adachi J, Aizawa K, Arakawa T, Fukuda S, Hara A, Hashizume W, Hayatsu N, Imotani K, Ishii Y, Itoh M, Kagawa I, Kondo S, Konno H, Miyazaki A, Osato N, Ota Y, Saito R, Sasaki D, Sato K, Shibata K, Shinagawa A, Shiraki T, Yoshino M, Hayashizaki Y, Yasunishi A.
Department of Molecular Biology, National Institute of Agrobiological Sciences, 2-1-2 Kannon-dai, Tsukuba, Ibaraki 305-8602, Japan. skikuchi@nias.affrc.go.jp
Erratum in:
- Science. 2003 Sep;301(5641):1849.
Comment in:
We collected and completely sequenced 28,469 full-length complementary DNA clones from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). Mapping of the cDNA clones to genomic DNA revealed that there are 19,000 to 20,500 transcription units in the rice genome. Protein informatics analysis against the InterPro database revealed the existence of proteins presented in rice but not in Arabidopsis. Sixty-four percent of our cDNAs are homologous to Arabidopsis proteins.
PMID: 12869764 [PubMed - indexed for MEDLINE]
Publication Types:
MeSH Terms:
- Alternative Splicing
- Amino Acid Sequence
- Cloning, Molecular
- Computational Biology
- DNA, Complementary
- Databases, Nucleic Acid
- Databases, Protein
- Genes, Plant
- Genome, Plant*
- Molecular Sequence Data
- Open Reading Frames
- Oryza sativa/genetics*
- Plant Proteins/chemistry
- Plant Proteins/genetics
- Plant Proteins/physiology
- Protein Structure, Tertiary
- RNA, Antisense/genetics
- Sequence Analysis, DNA*
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Transcription Factors/chemistry
- Transcription Factors/genetics
- Transcription, Genetic
Substances: