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    FEBS Lett. 1992 Dec 21;314(3):386-8.

    A 25 kDa alpha 2-microglobulin-related protein is a component of the 125 kDa form of human gelatinase.

    Triebel S, Bläser J, Reinke H, Tschesche H.

    Department of Biochemistry, Faculty of Chemistry, University of Bielefeld, Germany.

    Besides the monomeric mammalian 95 kDa progelatinase, two additional forms, a disulfide-bridged 220 kDa dimer and a 125 kDa form were isolated from human PMN leukocytes. The 125 kDa progelatinase was identified as a covalently linked, disulfide-bridged heterodimer formed of the monomer with a 25 kDa protein. This 25 kDa protein was isolated from gelatinase bound to the affinity support of gelatin-Sepharose and eluted by DTE-containing buffer. The amino acid sequence of tryptic peptides of this protein revealed homology with an alpha 2-microglobulin-related protein from rats, a protein so far unknown in humans.

    PMID: 1281792 [PubMed - indexed for MEDLINE]

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