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    Zhonghua Nan Ke Xue. 2003 Feb;9(1):24-7.

    [Molecular cloning of human sperm surface protein P34H gene and semi-quantitative analysis of its expression in testis and epididymidis]

    [Article in Chinese]

    Xia XY, Xu XF, Gao Y, Huang YF.

    College of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210097, China.

    OBJECTIVES: To identify, clone and sequence human sperm protein P34H gene and perform semi-quantitative analysis of its expression in testis and epididymidis. METHODS: Total RNA was prepared from human epididymidis tissue. cDNA fragment encoding human P34H was amplified by RT-PCR using specific primers, and then was cloned into pGEM-T vector. Inserted P34H gene was sequenced by ABI 377 DNA Sequencer. Meanwhile, semi-quantitative analysis of P34H expression in testis and caput, corpus, cauda epididymidis was done by RT-PCR based on beta-actin as an inner control. RESULTS: Human sperm protein P34H gene was successfully cloned and its cDNA sequence was submitted to GenBank(Accession No. AF515625). CONCLUSIONS: The cloning of P34H gene may be useful for the further basic and clinical studies on P34H.

    PMID: 12680326 [PubMed - indexed for MEDLINE]

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