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    Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003 Apr;20(2):107-10.

    [Molecular cloning of TSARG3 gene related to apoptosis in human spermatogenic cells]

    [Article in Chinese]

    Liu G, Lu G, Fu J, Liu S, Xing X, Li L.

    Human Reproductive Engineering Institute, Xiangya Medical School, Central South University, Changsha, Hunan, 410078 P. R. China. lgxdiredtor@sina.com

    OBJECTIVE: To clone a novel gene which is related to human testis spermatogenesis apoptosis. METHODS: To rapidly attain human novel gene full-length cDNA sequence from a human testis cDNA library,the gene-specific primers and the vector-specific primers were designed for nested polymerase chain reaction. Sequencing was performed and the result was analysed. RESULTS: The present authors discovered the TSARG3 gene(GenBank accession number AF419291) from a human testis cDNA library, using a cDNA fragment (GenBank accession number BE644537) as an electronic probe, which was significantly changed in cryptorchidism and represented a novel gene. Furthermore, a mouse homologue of this gene was identified (GenBank accession number AF419292) by using the same method. CONCLUSION: A novel gene named TSARG3 was cloned. It is considered that the function of the new gene is related to human testis spermatogenesis apoptosis.

    PMID: 12673577 [PubMed - indexed for MEDLINE]

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