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    FEMS Microbiol Lett. 2002 May 21;211(1):37-41.

    Glutamate dehydrogenase of Halobacterium salinarum: evidence that the gene sequence currently assigned to the NADP+-dependent enzyme is in fact that of the NAD+-dependent glutamate dehydrogenase.

    Hayden BM, Bonete MJ, Brown PE, Moir AJ, Engel PC.

    Department of Biochemistry and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland.

    A GDH gene from Halobacterium salinarum has been cloned and sequenced and the publication assigns the sequence to the NADP+-glutamate dehydrogenase of this organism. We have expressed this gene in Escherichia coli and find that it encodes an NAD+-dependent glutamate dehydrogenase without activity towards NADP+. Further, peptide sequence from the two corresponding proteins supports the view that the deposited sequence is indeed that of the NAD+-dependent glutamate dehydrogenase. Sequence from the NAD+-dependent protein matches the published gene sequence, whereas sequence from the NADP+ glutamate dehydrogenase does not.

    PMID: 12052548 [PubMed - indexed for MEDLINE]

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