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    Acta Crystallogr D Biol Crystallogr. 2002 May;58(Pt 5):846-8. Epub 2002 Apr 26.

    Cloning, high-level expression, purification and crystallization of peptide deformylase from Leptospira interrogans.

    Li Y, Ren S, Gong W.

    School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.

    A new peptide deformylase (PDF; EC 3.5.1.27) gene from Leptospira interrogans was identified and cloned into expression plasmid pET22b(+) and was highly expressed in Escherichia coli BL21(DE3). With DEAE-Sepharose anion-exchange chromatography followed by Superdex G-75 size-exclusion chromatography, 60 mg of PDF from L. interrogans was purified from 1 l of cell culture. Crystallization screening of the purified enzyme resulted in two crystal forms, from one of which a 3 A resolution X-ray diffraction data set has been collected.

    PMID: 11976499 [PubMed - indexed for MEDLINE]

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