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    FEBS Lett. 2001 Oct 19;507(1):21-4.

    Cingulin interacts with F-actin in vitro.

    D'Atri F, Citi S.

    Department of Molecular Biology, University of Geneva, Geneva 4, Switzerland.

    Cingulin, a M(r) 140-160 kDa protein of the cytoplasmic plaque of epithelial tight junctions (TJ), interacts in vitro with TJ proteins and myosin. Here we investigated cingulin interaction with actin, using His-tagged, full-length Xenopus laevis cingulin expressed in insect cells, and glutathione S-transferase (GST) fusion proteins of fragments of cingulin expressed in bacteria. Purified full-length cingulin co-pelleted with F-actin after high speed centrifugation, and promoted the sedimentation of F-actin under low speed centrifugation, suggesting that cingulin is an actin-cross-linking protein. The actin interaction of GST fusion proteins containing fragments of Xenopus cingulin suggested that the F-actin binding site is between residues 101 and 294.

    PMID: 11682052 [PubMed - indexed for MEDLINE]

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