Analytical Research & Development Department, Amgen Inc., Thousand Oaks, California 91320, USA.
The disulfide structure of alfimeprase, a recombinant analog of fibrolase, was experimentally determined by a combination of peptide mapping, Edman degradation, and mass spectrometry. The three disulfide bonds were determined to be Cys-116/196, Cys-156 /180, and Cys-158/163 with the residue number system of alfimeprase.