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    Gene. 2001 Feb 21;264(2):187-96.

    Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene.

    Dong H, Lin W, Zhang CK, Xiong H, Fu G, Jin WR, Chen R, Chen Z, Qi ZT, Huang GM.

    Chinese National Human Genome Center at Shanghai, 351 Guo Shou Jing Road, Zhangjiang High Tech Park, Shanghai 201203, P.R. China.

    Chromatin assembly factor-1 (CAF-1) plays essential roles in eukaryotic chromatin assembly during DNA replication (Smith and Stillman, 1989. Cell 58, 15-25), (Krude, 1999. Eur. J. Biochem. 263, 1-5). Its p150 subunit, involved in interaction with histone H3 and H4, is critical to the CAF-1 nucleosome assembly activity. In this study, we sequenced a 96-kb genomic DNA region that includes a 42.8-kb CAF-1 p150 subunit gene (CHAF1A), and a 41.1-kb EEN gene. A scripted bioinformatics analysis pipeline (research agent) has been set up to annotate the BAC sequence with a set of integrated algorithms. The CAF-1 p150 subunit gene contains 15 exons and 14 introns. The promoter region is characterized by deletional analyses, revealing a potential repressor. Tissue-correlated alternative splicing forms of the transcript was initially identified by EST clustering analysis, then confirmed by RT-PCR which resulted more splicing forms than computational prediction.

    PMID: 11250073 [PubMed - indexed for MEDLINE]

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