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    Biochem Biophys Res Commun. 2000 Apr 29;271(1):164-9.

    Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.

    Hino M, Kurogi K, Okubo MA, Murata-Hori M, Hosoya H.

    Department of Biological Science, Faculty of Science, Hiroshima University, Higashi-Hiroshima, 739-8526, Japan.

    One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both alpha- and beta-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells. Copyright 2000 Academic Press.

    PMID: 10777697 [PubMed - indexed for MEDLINE]

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