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    EMBO J. 2000 Apr 3;19(7):1494-504.

    GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28.

    Sagiv Y, Legesse-Miller A, Porat A, Elazar Z.

    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 76100 Israel.

    Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.

    PMID: 10747018 [PubMed - indexed for MEDLINE]

    PMCID: 310219

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