Berkeley Drosophila Genome Project, Howard Hughes Medical Institute, and Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA.
Collections of nonredundant, full-length complementary DNA (cDNA) clones for each of the model organisms and humans will be important resources for studies of gene structure and function. We describe a general strategy for producing such collections and its implementation, which so far has generated a set of cDNAs corresponding to over 40% of the genes in the fruit fly Drosophila melanogaster.