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    J Hum Genet. 1999;44(3):185-9.

    Cloning, tissue expression, and chromosomal assignment of human MRJ gene for a member of the DNAJ protein family.

    Seki N, Hattori A, Hayashi A, Kozuma S, Miyajima N, Saito T.

    Genome Research Group, National Institute of Radiological Sciences, Chiba, Japan.

    The DnaJ protein family consists of proteins with a highly conserved amino acid stretch called the "J-domain". A cDNA clone encoding a new protein with a J-domain was isolated from a human fetal brain cDNA library. This new member of the DnaJ family of 241 amino acid residues showed 94% identity with mouse Mrj (accession number, AF035962) and 71% identity with mouse Msj-1 (accession number, U95607) along its entire sequence. Reverse transcription-coupled polymerase chain reaction (RT-PCR) analysis showed the messenger RNA was ubiquitously expressed in various human tissues. The chromosomal location of the gene was determined by PCR-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel to map on chromosome 11q25 region.

    PMID: 10319584 [PubMed - indexed for MEDLINE]

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