Tdg thymine DNA glycosylase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tdgprovided by MGI
Official Full Name: thymine DNA glycosylaseprovided by MGI
Primary source: MGI:MGI:108247
See related: Ensembl:ENSMUSG00000034674 AllianceGenome:MGI:108247
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Jza1; JZA-3; E130317C12Rik
Summary: Enables several functions, including SUMO binding activity; protein kinase C binding activity; and transcription coregulator activity. Involved in DNA metabolic process; regulation of embryonic development; and regulation of gene expression, epigenetic. Acts upstream of or within negative regulation of chromatin binding activity; negative regulation of protein binding activity; and negative regulation of transcription by RNA polymerase II. Located in PML body. Is expressed in several structures, including epithelium; genitourinary system; nervous system; retina; and skin. Orthologous to human TDG (thymine DNA glycosylase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 41.6), CNS E14 (RPKM 32.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 C1; 10 39.72 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (82465649..82486633)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (82629814..82650799)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Thymine DNA glycosylase regulates cell-cycle-driven p53 transcriptional control in pluripotent cells.
Title: Thymine DNA glycosylase regulates cell-cycle-driven p53 transcriptional control in pluripotent cells.
TET1 and TDG Suppress Inflammatory Response in Intestinal Tumorigenesis: Implications for Colorectal Tumors With the CpG Island Methylator Phenotype.
Title: TET1 and TDG Suppress Inflammatory Response in Intestinal Tumorigenesis: Implications for Colorectal Tumors With the CpG Island Methylator Phenotype.
Roles of TET and TDG in DNA demethylation in proliferating and non-proliferating immune cells.
Title: Roles of TET and TDG in DNA demethylation in proliferating and non-proliferating immune cells.
These findings suggest that the catalytic and scaffolding activities of TDG are essential for retinoic acid-dependent gene expression and provide important insights into the mechanisms underlying targeting of TET-TDG complexes.
Title: Regulation of Active DNA Demethylation through RAR-Mediated Recruitment of a TET/TDG Complex.
The DNA demethylation marks are dynamically regulated in both in vivo and in vitro aging conditions, which are associated with Tet3 over-expression and Tdg repression.
Title: Dynamic changes of DNA epigenetic marks in mouse oocytes during natural and accelerated aging.
TDG, as a new coactivator, promotes beta-catenin/TCFs transactivation and functionally cooperates with CBP in canonical Wnt signaling.
Title: Thymine DNA glycosylase promotes transactivation of β-catenin/TCFs by cooperating with CBP.
Embryonic lethality in Tdg (-/-) embryos is due, in part, to the reduction of noradrenaline levels.
Title: Embryonic lethality in mice lacking mismatch-specific thymine DNA glycosylase is partially prevented by DOPS, a precursor of noradrenaline.
data suggest that oxidation of 5-methylcytosine by Tet proteins followed by TDG-mediated base excision of 5-carboxylcytosine constitutes a pathway for active DNA demethylation
Title: Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA.
Results demonstrate that TDG is required for normal mammalian development and the establishment of proper promoter/enhancer DNA methylation patterns that are conducive to transcription during embryogenesis
Title: Thymine DNA glycosylase is essential for active DNA demethylation by linked deamination-base excision repair.
TDG-dependent DNA repair has evolved to provide epigenetic stability in lineage committed cells
Title: Embryonic lethal phenotype reveals a function of TDG in maintaining epigenetic stability.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (5) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Tdg-ps1 (e-PCR), detects polymorphism
- UniSTS:531492

RH124550 (e-PCR)
- UniSTS:161372

Tdg (e-PCR), detects polymorphism
- UniSTS:144584

Tdg-ps1 (e-PCR), detects polymorphism
- UniSTS:144585

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables DNA N-glycosylase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA N-glycosylase activity	ISO Inferred from Sequence Orthology more info
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables G/T mismatch-specific thymine-DNA glycosylase activity	IEA Inferred from Electronic Annotation more info
enables G/U mismatch-specific uracil-DNA glycosylase activity	ISO Inferred from Sequence Orthology more info
enables SUMO binding	IPI Inferred from Physical Interaction more info	PubMed
enables SUMO binding	ISO Inferred from Sequence Orthology more info
enables chloride ion binding	ISO Inferred from Sequence Orthology more info
enables double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables mismatch base pair DNA N-glycosylase activity	IEA Inferred from Electronic Annotation more info
enables mismatched DNA binding	ISO Inferred from Sequence Orthology more info
enables nucleic acid binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein kinase C binding	IPI Inferred from Physical Interaction more info	PubMed
enables pyrimidine-specific mismatch base pair DNA N-glycosylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables pyrimidine-specific mismatch base pair DNA N-glycosylase activity	ISO Inferred from Sequence Orthology more info
enables sodium ion binding	ISO Inferred from Sequence Orthology more info
enables transcription coregulator activity	IGI Inferred from Genetic Interaction more info	PubMed
enables uracil DNA N-glycosylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables uracil DNA N-glycosylase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA repair	IEA Inferred from Electronic Annotation more info
involved_in base-excision repair	IDA Inferred from Direct Assay more info	PubMed
involved_in base-excision repair	ISO Inferred from Sequence Orthology more info
involved_in base-excision repair, AP site formation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in epigenetic regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of chromatin binding	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of protein binding	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in regulation of embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in PML body	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: G/T mismatch-specific thymine DNA glycosylase

Names: C-JUN leucine zipper interactive protein JZA-3

NP_001345446.1

EC 3.2.2.29

NP_035691.2

EC 3.2.2.29

NP_766140.2

EC 3.2.2.29

XP_030100908.1

EC 3.2.2.29

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001358517.2 → NP_001345446.1 G/T mismatch-specific thymine DNA glycosylase isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 2. Isoform 3 has the same N- and C-termini, but is one amino acid shorter than isoform 2.

Source sequence(s)

AC152980

Conserved Domains (1) summary

TIGR00584
Location:2 → 340

mug; mismatch-specific thymine-DNA glycosylate (mug)
NM_001428834.1 → NP_001415763.1 G/T mismatch-specific thymine DNA glycosylase isoform 4

Status: VALIDATED

Source sequence(s)

AC152980
NM_001428835.1 → NP_001415764.1 G/T mismatch-specific thymine DNA glycosylase isoform 5

Status: VALIDATED

Source sequence(s)

AC152980
NM_001428836.1 → NP_001415765.1 G/T mismatch-specific thymine DNA glycosylase isoform 6

Status: VALIDATED

Source sequence(s)

AC152980
NM_001428837.1 → NP_001415766.1 G/T mismatch-specific thymine DNA glycosylase isoform 7

Status: VALIDATED

Source sequence(s)

AC152980
NM_001428838.1 → NP_001415767.1 G/T mismatch-specific thymine DNA glycosylase isoform 8

Status: VALIDATED

Source sequence(s)

AC152980
NM_011561.4 → NP_035691.2 G/T mismatch-specific thymine DNA glycosylase isoform 1

See identical proteins and their annotated locations for NP_035691.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) lacks an alternate in-frame segment in the 5' coding region and uses a downstream start codon, compared to variant 2. Isoform 1 has a shorter N-terminus, compared to isoform 2.

Source sequence(s)

AC152980

Consensus CDS

CCDS36013.1

UniProtKB/Swiss-Prot

P56581

Related

ENSMUSP00000089917.5, ENSMUST00000092266.11

Conserved Domains (1) summary

TIGR00584
Location:2 → 316

mug; mismatch-specific thymine-DNA glycosylate (mug)
NM_172552.5 → NP_766140.2 G/T mismatch-specific thymine DNA glycosylase isoform 2

See identical proteins and their annotated locations for NP_766140.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).

Source sequence(s)

AC152980

Consensus CDS

CCDS24070.1

UniProtKB/Swiss-Prot

P56581, Q3TPW4, Q542A9, Q5U3M4, Q5U3M5, Q6PJW4, Q8BPK0

Related

ENSMUSP00000121000.2, ENSMUST00000151390.8

Conserved Domains (1) summary

TIGR00584
Location:2 → 340

mug; mismatch-specific thymine-DNA glycosylate (mug)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

82465649..82486633

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030245048.2 → XP_030100908.1 G/T mismatch-specific thymine DNA glycosylase isoform X1

Conserved Domains (2) summary

TIGR00584
Location:2 → 316

mug; mismatch-specific thymine-DNA glycosylate (mug)

cd10028
Location:125 → 129

UDG-F2_TDG_MUG; motif A [structural motif]