Gene Ther. 1998 Nov;5(11):1499-507.

Successful use of a plant gene in the treatment of cancer in vivo.

Cortés ML, de Felipe P, Martín V, Hughes MA, Izquierdo M.

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Departamento de Bioquímica y Biología Molecular-Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Facultad de Ciencias, Spain.

Abstract

A new strategy for cancer gene therapy has been developed using a plant gene which encodes the enzyme, linamarase, that hydrolyzes the cyanogenic glucoside substrate, linamarin, into glucose, acetone and cyanide. Retroviral vectors that carry linamarase as a potential killer-suicide gene cause a marked sensitization to the innocuous substrate, linamarin, followed by cell death. We show that the system can eradicate very large intracerebral gliomas in vivo helped by a cyanide bystander effect. Animals showing a total regression of the tumor by magnetic resonance imaging (MRI), do not show other appreciable toxic effects.

PMID:: 9930303; [PubMed - indexed for MEDLINE]

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Successful use of a plant gene in the treatment of cancer in vivo.

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